We observed a variability in oocysts counts when different antibodies where used with laser scanning cytometers and manual microscopy. Highly significant correlations were obtain between both cytometers (R 2 = 0.99) and with manual microscopy (R 2 = 0.98), showing that oocysts counts made by cytometers were equivalent to those obtained with conventional methods. Two mAbs from commercial suppliers were used to evaluate background reduction. Reference material and surface water samples were analysed by two laser scanning cytometers methodologies and by manual epifluorescence microscopy. The aim of this study was to validate and compare the capacity of two laser scanning cytometers commercially available (LSCĀ® and ChemScanRDIĀ®), against manual microscopic enumeration of Cryptosporidium oocysts in surface water and reference material samples. Cryptosporidium detection in water and environmental samples has increased during the last years, largely due to an increase in the number of reported waterborne outbreaks of cryptosporidiosis and the implementation of new regulations about Cryptosporidium monitoring in water supplies.
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